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Figure 2 | Cell & Bioscience

Figure 2

From: Suppression of autophagy by chloroquine sensitizes 5-fluorouracil-mediated cell death in gallbladder carcinoma cells

Figure 2

Effect of CQ in 5-FU-induced GBC cell proliferation and mortality inhibition. The effect of CQ-pretreatment on the inhibitory effect of 5-FU on the proliferative activity and cell mortality rate of SGC-996 cells and GBC-SD cells was investigated by CCK-8 assay and trypan blue exclusion staining. The y-axis represents the proliferation/mortality rate, calculated as the ratio to normal control (untreated cells). Values were given as mean ± SD. (A) Pre-treatment of cells with CQ at 100 μM for 12 hours prior to exposure to 5-FU at 5 μM for 48 hours, resulted in significantly potentiation of the inhibitory effect (25% vs. 62% inhibition (SGC-996 cells) and 24% vs. 50% inhibition (GBC-SD cells) for 5-FU alone and CQ + 5-FU, respectively) (*,p < 0.05 vs. control, n = 5). (B) When cells were treated with CQ at 100 μM for 12 hours prior to exposure to 5-FU at 5 μM for 48 hours, the mortality rate reduction was notably potentiated (60% vs. 28% live cells (SGC-996 cells) and 67% vs. 21% live cells (GBC-SD cells) for 5-FU alone and CQ + 5-FU , respectively) (*,p < 0.05 vs. control, n = 5).

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