Figure 6
Characterization of OVA-antigen specific CD8+ T cell in the tumor infiltrating lymphocytes derived from the spontaneous ovarian tumors following intra-peritoneal injection of chimeric NKG2D-Fc-RO protein. 10-week-old tumor-bearing MISIIR mice were treated with 20 μg/mouse of NKG2D-Fc or NKG2D-Fc-RO protein every week for four weeks and 2.5x106 OT-1 CD8+ T cells were injected intraperitoneally every other week two times. Mice were sacrificed 1 week after the last treatment. Ovarian tumors were harvested and tumor infiltrating lymphocytes were characterized for OVA-specific CD8+ T cells using OVA peptide-loaded H-2Kb tetramer staining. A. Representative flow cytometry analysis for the characterization of OVA-specific CD8+ T cells in the tumors. B. Bar graph depicting the percentage of OVA-specific CD8+ T cells in total TILs (mean ± SD).