Figure 2

Characterization of the binding of NKG2D-Fc and NKG2D-Fc-RO to NKG2D ligand expressed by MOVCAR tumor cells using flow cytometry. (A) Representative flow cytometry analysis of antibody bound to Rae-1 (clear histogram) or isotype (shaded histogram) with B16F10 (negative control) and MOVCAR cells. (B) Purified proteins Fc (shaded histogram), NKG2D-Fc (clear histogram) and NKG2D-Fc-RO (clear histogram) were incubated with either B16F10 or MOVCAR cells and then stained with phycoerythrin (PE)-labeled antimouse Fc antibody.