Figure 2
From: Resveratrol induces insulin gene expression in mouse pancreatic α-cells
Resveratrol induced insulin levels are enhanced further by HDAC inhibition. (A) ChIP-qPCR analysis showing H3/H4 acetylation at Ins2 promoter after resveratrol alone (25 μM / 24 hr) or TSA (50 nM) co-treatment in αTC9. (B) qPCR analysis of Ins2 mRNA after resveratrol alone (25 μM / 24 hr) or TSA (50 nM / 24 hr) co-treatment in αTC9. (C) qPCR analysis of Ins2 mRNA after resveratrol alone (25 μM / 24 hr) or TSA (50 nM / 24 hr) co-treatment in INS-1 cells. (D) RIA measurement of intracellular insulin protein after resveratrol (25 μM / 24 hr) and TSA (50 nM / 24 hr) co-treatment in α-cells. Bars represent the mean of the respective individual ratios ± SEM (n = 3). The asterisk indicates P < 0.05.