Figure 7
Distinct expression profiles of HAL1 and HAL2 during development. Total RNA isolated from whole animals from embryonic stage 30 to the end of metamorphosis (stage 66) was subjected to quantitative RT-PCR analysis for HAL1 and HAL2 gene expression. Note that HAL1 was expressed strongly in embryos and repressed after stage 45 when tadpole feeding begins while HAL2 was upregulated by stage 45 and its expression continued to rise till the end of metamorphosis.