17-DMAG upreguateded Lgr5 expression in crypt cells. (A) Lgr5 was detected with Western blot and its expression increased in intestines of 17-DMAG-treated mice compared to vehicle-treated animals 30 days after irradiation at 8.75-Gy. (B) Densitometric quantitation of Lgr5 protein.* p < 0.01 vs. Veh/RI group, error bars indicating the SEM for three or four independent experiments. Lgr5 (indicated by white arrows) localizing at base cells of crypts in vehicle-pretreated (C) and 17-DMAG-pretreatd mice (D) was demonstrated using immune-staining method and confocal microscope (20 ×) 30 days after irradiation and a 100 μm scale bar was shown on the bottom. N = 3–4 mice per group.