Figure 4
17-DMAG attenuated bone marrow damage after irradiation. Morphological alteration in femur bone marrows of mice pretreated with (A) vehicle + sham, (B) 17-DMAG + sham, (C) vehicle + RI, and (D) 17-DMAG + RI 30 days after irradiation at 8.75 Gy. The images were from epiphyseal ends with H & E staining, 20 × magnification. A 100 μm scale bar was shown on the bottom of right panel D. Radiation induced vast reduction of hematopoietic cellularity and replacement by adipocytes in bone marrow. However, (E) a significantly improved number of megakaryocytes and (F) a significantly decreased numbers of adipocytes were found per field under microscopy (20 ×) in bone marrows of 17-DMAG-pretreated mice compared to those in vehicle-pretreated group 30 days after irradiation. * p < 0.001 vs. Veh/sham, 17-D/sham, and 17-D/RI; ** p < 0.01 vs. Veh/Sham, 17-D/sham, and Veh/RI group. Error bars indicate the SEM for at least 4–5 independent experiments. Veh: vehicle; 17-D: 17-DMAG; RI: radiation injury.