In vivo tumor protection experiments. (A) An outline of the vaccination schedule using either pcDNA3-MCC/ST DNA vaccine or empty vector control vaccine pcDNA3. C57BL/6 mice (5 per group) were immunized with either pcDNA3-MCC/ST or pcDNA3 DNA vaccine intradermally with DNA-coated particles using a helium-driven gene gun 3 times at 4-day intervals. Ten days after the last vaccination, vaccinated mice were challenged subcutaneously in the right flank with B16/ST tumor (1×105 cells/mouse). (B) Survival plot depicting the percentage of vaccinated mice surviving following vaccination with either pcDNA3-MCC/ST DNA vaccine or empty vector control pcDNA3. (C) Plot depicting tumor volume over time. Tumors were measured with digital calipers and tumor volumes calculated.