Figure 8

Antioxidant attenuated autophagy inhibition induced aggravation of ischemic liver injury in vivo . The rats were treated as indicated. (A and B) Levels of MDA (A) and T-AOC (B) of rat livers were detected. Data were shown as mean ± SEM (n = 6; *: p < 0.05; **: p < 0.01). (C) MitoSOX Red staining positive cells of liver cryosections were identified by fluorescence microscopy (left panel) and quantified (right panel). Data were shown as mean ± SEM (n = 3; * p < 0.05). (D) Mitochondrial membrane potential of rat livers were detected by Rho123 staining and analyzed by FACScan flowcytometry. Data were shown as mean ± SEM (n = 3; * p < 0.05). (E) Images were representative H&E staining of the liver sections with an original magnification × 200. (F and G) Serum levels of ALT (F) and AST (G) of rats were detected. Data were shown as mean ± SEM (n = 6; *: p < 0.05; **: p < 0.01).