Figure 4
From: Monoubiquitination of EEA1 regulates endosome fusion and trafficking
Expression of Ub-EEA1 alters Golgi morphology and induces fusion of lysosome with endosome. COS7 cells expressing Ub-EEA1 were fixed and immunostained with EEA1 antibody (B, E, H, K) together with antibodies against β-COP (A), PDI (D), Tom20 (G), or Lamp1 (J). Cells were also stained with DAPI in blue to reveal the nuclei. Merged two-color images are shown in C, F, I, L. Scale bars correspond to 20 μm.