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Table 7 Colony PCR program in Golden Gate cloning round 2

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Cycle number Temperature (°C) Time (min)
1 95 5
2-31 94 0.5
  52 0.5
  72 0.5
32 72 10
33 4 ~