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Table 5 Ligation mix in Golden Gate cloning round 2

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Components pCS2-TALEN Mix (μL)
Buffer 3 (NEB) 1
pFUS_A-RVDs vectors (150 ng/μL) 0.8
pFUS_B-RVDs vectors (150 ng/μL) 0.8
Last Repeats vectors (150 ng/μL) 0.6
BsmbI (NEB) 0.8
pCS2-TALEN-ELD/KKR vectors (150 ng/μL) 1
dH2O 5
Total 10