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Table 4 Colony PCR program in Golden Gate cloning round 1

From: Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos

Cycle number

Temperature (°C)

Time (min)

1

95

5

2-31

94

0.5

 

52

0.5

 

72

1.5

32

72

10

33

4

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