Figure 3

Nucleosome remodeling by hSWI/SNF converts B-DNA to Z-DNA. a. Mononucleosomes, assembled as described in Figure 2a, were incubated with hSWI/SNF with or without ATP for 30 minutes at 30°C. ZaaFok was added as indicated and reactions were incubated for an additional 30 minutes at room temperature. Reactions were stopped by adding Proteinase K and SDS. Cleavage by Zaa-Fok was monitored on a native polyacrylamide gel. Arrow heads indicate cleavage products. b. The affect of hSWI/SNF on Z-DNA formation on naked DNA was monitored. The conditions used in 3a. were repeated on the DNA templates. c. Restriction enzyme accessibility reveals different remodeling patterns between T140 and Z140 or B140 mononucleosomes. Mononucleomes assembled as in Fig. 2a were incubated with hSWI/SNF (with or without ATP) for 30 minutes at 30°C followed by 60 minute digestion with the indicated restriction enzymes. Cleavage bands were quantitated on native polyacrylamide gel and plotted to reflect changes in cleavage patterns due to hSWN/SNF remodeling.