Examination of the markers of PGCs and oocytes by immunofluorescent staining. (A) Time course immunofluorescence analysis of the expression of Nanos2, Nanos3, Nanog, Blimp1, and Nobox, the percentage of positive cells for each corresponding marker at different days of culture was statistized. Data are means ± SE. (n = 4). (B-D) Co- immunofluorescence of Blimp1/Nobox (B), Blimp1/Stella (C), and GDF9/Nobox (D) in SSCs-derived oocyte.