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Figure 5 | Cell & Bioscience

Figure 5

From: Histone H3K79 methyltransferase Dot1L is directly activated by thyroid hormone receptor during Xenopus metamorphosis

Figure 5

The activation of Xenopus tropicalis Dot1L promoter by liganded TR in vivo is dependent on TRE2. On the top of each panel shows a schematic diagram of the promoter construct with or without mutations in TRE1 (mTRE1) and/or TRE2 (mTRE2). The lower portion shows the promoter activity in the frog oocytes. For the transcription assay, wild type or mutant promoter construct was co-injected with the control Renilla luciferase construct phRG-tk into the nuclei of the oocytes with or without prior cytoplasmic injection of mRNAs for Xenopus tropicalis TRα and RXRβ. The oocytes were incubated at 18°C overnight in the presence or absence of 100 nM T3 and then used for dual luciferase assays. The relative activities of the firefly luciferase to Renilla luciferase were plotted. Error bars indicate s.e.m. Note that mutation of TRE1 alone had no effect on the regulation of the promoter by TR/RXR both in the presence or absence of T3 while mutating of TRE2 alone or together with TRE1 abolished the regulation by TR/RXR, suggesting that TRE2 is the functional TRE

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