Upregulation of Xenopus tropicalis Dot1L expression in the intestine and tail during natural metamorphosis. Total RNA was isolated from the intestine (A) and tail (B) at the indicated developmental stages from premetamorphosis to the end of metamorphosis (stage 66). The expression of Dot1L mRNA was determined by qRT-PCR as in Figure 2. Error bars indicate s.e.m. Note that there were no tail samples at stage 66 (B) as the tail is completely resorbed by stage 66.