Figure 2

Xenopus tropicalis Dot1L is induced in the intestine and tail upon treatment of premetamorphic tadpoles with T3. Stage 54 tadpoles were treated with 10 nM T3 for 2 days and total RNA was isolated from the intestine (A) and tail (B). qRT-PCR was conducted to examine the expression of Dot1L mRNA. Dot1L expression was normalized to the expression of the control gene EF1α (elongation factor 1α). Error bars indicate s.e.m.