Figure 4

Enhanced nuclear expression of STAT3/4 and RelA proteins and augmented production of Th1 and Th17 cytokines in PDLIM2 deficient Teff cells. Splenic T cells from day 10 PLP_180–199-immunized PDLIM2^+/+ (WT) or PDLIM2^−/− (KO) mice were subjected to QRT-PCR to detect the relative expression levels of the indicated cytokines genes (A) or ELISA to detect the nuclear expression levels of STAT3, STAT4 and RelA (B). The expression levels of the indicated genes and proteins were represented as fold induction relative to their WT controls. C) Naive PDLIM2^−/− or PDLIM2^+/+ CD4⁺ Th cells were stimulated for the indicated time points with anti-CD3/anti-CD28 under Th1 or Th17 polarizing condition, followed by ELISA to detect the nuclear expression levels of STAT3 (in response to Th17 stimulation), STAT4 and RelA (in response to Th1 stimulation). In A-C, n = 3, *, p < 0.03; **, p < 0.003 by two tailed t-test.