PAT-9 is associated with gene promoters in vivo. ChIP experiments show enrichment of the indicated 5′ promoter DNA with the αPAT-9 antibody compared with NRS control as assayed by qPCR. The tbx-2 and daf-3 bars represent the results from three independent ChIP experiments and the frg-1 bar represents the results from six independent ChIP experiments. All qPCRs were performed as five replicates to generate one data point. Fold enrichment was calculated as 2e (Ct NRS – Ct PAT-9).