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Figure 2 | Cell & Bioscience

Figure 2

From: Unstabilized DNA breaks in HTLV-1 Tax expressing cells correlate with functional targeting of Ku80, not PKcs, XRCC4, or H2AX

Figure 2

Cytogenetic effects in hamster and mouse cells. a. Frequency of micronuclei (MN) in hamster (CHO, xrs-6) and mouse (DNA PKcs+/+, DNA PKcs−/−, XRCC4+/+, XRCC4−/−) cells transfected with the GFP or GFP-Tax expression plasmids. xxx: significantly different (P < 0.001) compared to values found in the respective control cells, made up of the same type of cells transfected with the GFP plasmid. x: significantly different (P < 0.05) compared to values found in the respective xrs-6 cells transfected with the GFP plasmid. °°°: significantly different (P < 0.001) compared to values found in the respective wild type control cells transfected with the GFP expression plasmid. □□: significantly different (P < 0.01) compared to values found in the respective XRCC4+/+ cells transfected with the GFP plasmid. b. Frequency of incorporation of DIG-dUTP in nuclei of hamster (CHO, XRS-6) and mouse (DNA PKcs+/+, DNA PKcs−/−, XRCC4+/+, XRCC4−/−) cells transfected with the GFP or GFP-Tax expression plasmid. xxx: significantly different (P < 0.001) compared to values found in the respective control cells, made up of the same type of cells transfected with the GFP plasmid. °°°: significantly different (P < 0.001) compared to values found in CHO cells transfected with the GFP plasmid. °°: significantly different (P < 0.01) compared to values found in the XRCC4+/+ cells transfected with the GFP plasmid. c. Frequency of MN in H2AX+/+ and H2AX−/− cells showing GFP or GFP-Tax proteins. xxx: significantly different (P < 0.001) compared to values found in the respective control cells, transfected with the GFP expression plasmid. °°°: significantly different (P < 0.001) compared to values found in the respective wild type control cells transfected with the GFP expression plasmid. d. Frequency (%) of DIG-dUTP incorporation in nuclei of H2AX+/+ and H2AX−/− cells showing GFP or GFP-Tax proteins. xxx: significantly different (P < 0.001) compared to values found in H2AX+/+ cells, transfected with the GFP plasmid. xx: significantly different (P < 0.01) compared to values found in H2AX−/− cells transfected with the GFP plasmid. °°°: significantly different (P < 0.001) compared to values found in H2AX+/+ cells transfected with the GFP plasmid.

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