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Figure 5 | Cell & Bioscience

Figure 5

From: Extracellular domain of CD98hc is required for early murine development

Figure 5

ΔCD98hc-β geo fusion protein interacts with integrin β1 similarly as wild type CD98hc. A. FLAG-tagged integrin β1 and wild type CD98hc or ΔCD98hc-β geo fusion protein were expressed in frog oocytes. Immunostaining of the sectioned oocytes with anti-integrin β1 antibody verified the cell surface expression (green) of FLAG-tagged integrin β1 protein. B. Co-immunoprecipitation analysis was performed with frog oocytes that expressed FLAG-tagged integrin β1 and wild type CD98hc or ΔCD98hc-β geo fusion protein. Anti-FLAG antibody immunoprecipitated FLAG-integrin β1 as expected and also co-immunoprecipitated CD98hc and ΔCD98hc-β geo fusion protein with similar efficiencies. C. Co-immunoprecipitation analysis was performed with PST080 ES cells. Anti-integrin β1 antibody pulled down ΔCD98hc-β geo fusion protein or CD98hc together with endogenous integrin β1.

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