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Figure 5 | Cell & Bioscience

Figure 5

From: In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination

Figure 5

Characterization of the number of E7-specific IFN-γ CD8+ T cells in mice vaccinated intradermally with CRT/E7 DNA in combination with bic155, I155, or a control construct. C57BL/6 mice (3 per group) were immunized with CRT/E7 in combination with bic155, I155, or a control construct. At day 7, animals received boosters at the same dose and regimen. At day 14, splenocytes were harvested, cultured for 15 hrs with (black bars) or without (grey bars) E7 peptide, and stained for surface CD8 and intracellular IFN-γ. Cells were analyzed by flow cytometry. A, Representative flow cytometry data depicting the number of E7-specific CD8+ T cells. B, Bar graph representation of the flow cytometry data (mean ± SD). The y-axis on the bar graph depicts the number of IFN-γ-secreting E7-specific CD8+ T cells per 3 × 105 counted splenocytes (mean ± SD).

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