Characterization of the number of E7-specific IFN-γ CD8+ T cells in mice vaccinated intradermally with CRT/E7 DNA in combination with either bic155or a control construct. A, C57BL/6 mice (3 per group) were immunized with CRT/E7 DNA in combination with either bic155(left panels) or a control construct (right panels). At day 7, animals received boosters at the same dose and regimen. At day 14, splenocytes were harvested, cultured for 15 hrs with (top panels) or without (bottom panels) E7 peptide, and stained for surface CD8 and intracellular IFN-γ. Cells were analyzed by flow cytometry. The top right quadrant in each dot plot indicates the number of IFN-γ-secreting E7-specific CD8+ T cells. B, Bar graph representation of the flow cytometry data (mean ± SD).