Figure 5
From: Mutual regulation between deubiquitinase CYLD and retroviral oncoprotein Tax
A phospho-mimetic CYLD is defective in inhibiting Tax ubiquitination. HEK293 cells were transfected with Tax and HA-ubiquitin either in the absence (-) or presence of wildtype (WT) CYLD, the catalytically inactive CYLD mutant (Mut), or CYLD mutants harboring serine-to-alanine (SA) or serine-to-glutamic acids (SE) substitutions at its phsophorylation sites. Cell lysates were subjected to Tax ubiquitination (upper) or direct IB to analyze the expression of ubiquitin, CYLD, and Tax (lower three panels).