In vivo tumor treatment experiment with HPV-16 pseudovirion vaccination. 5-8 weeks old C57BL/6 mice (5 mice per group) were injected with 1 × 105 B16/OVA tumor cells subcutaneously. 3 days after tumor cell injection, the mice were vaccinated with either 5 μg (total L1 protein amount) of HPV-16/OVA, or HPV-16/GFP pseudovirions via footpad injection. The mice were boosted with the same regimen on day 10 and 17. Tumor growth was monitored twice a week and tumor volume was calculated as described in the Material and Methods section.