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Figure 1 | Cell & Bioscience

Figure 1

From: Distinct biological effects of different nanoparticles commonly used in cosmetics and medicine coatings

Figure 1

Treatment with ZnO, TiO 2 , and FeO did not activate caspase-3 or caspase-9. A, HT29-SCAT3 cells were treated with different nanoparticles as indicated (ZnO 10 = 10 μg/ml, ZnO 20 = 20 μg/ml, TiO2 10 = 10 μg/ml, TiO2 30 = 30 μg/ml, TiO2 100 = 100 μg/ml, FeO 10 = 10 μg/ml, FeO 30 = 30 μg/ml, FeO 100 = 100 μg/ml). Staurosporine (STS = 1 μM) was used as a positive control to induce caspase-3 activation. The images were analyzed using an automated ImageXpress Micro microscope at 20× magnification. The changes in the emission ratio after treatment and exposure to 435 nm light (530 nm ECFP/475 nm Venus) were measured as described by Takemoto et al. 2003 and quantitated using the MetaXpress software. This experiment was repeated 3 times. B, HT29-SCAT9 cells were treated with different nanoparticles as indicated (ZnO 10 = 10 μg/ml, ZnO 20 = 20 μg/ml, TiO2 10 = 10 μg/ml, TiO2 30 = 30 μg/ml, TiO2 100 = 100 μg/ml, FeO 10 = 10 μg/ml, FeO 30 = 30 μg/ml, FeO 100 = 100 μg/ml). Staurosporine (STS = 1 μM) was used as a positive control to induce caspase-9 activation. The images were analyzed using an automated ImageXpress Micro microscope at 20× magnification. The changes in the emission ratio after treatment and exposure to 435 nm light (530 nm ECFP/475 nm Venus) were measured as described by Takemoto et al. 2003 and quantitated using the MetaXpress software. This experiment was repeated 3 times.

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