TIMP-1-/- mice have greater liver fibrosis than wild-type mice post 4-week-CCl4 treatment. Mice were treated with CCl4 for 4 weeks and euthanized 24 hours following the last injection. A, B, Liver tissues were collected for Sirius red staining (A) and immunohistochemical staining with α-SMA antibodies (B). The surface areas stained with Sirius red or α-SMA were quantified and shown in the right panel. C, Western blot analyses of α-SMA from liver tissue protein extracts. The values represent values ± SEM (n = 5-8). *P < 0.05.