Systematic analysis of secreted proteins reveals synergism between IL6 and other proteins in soft agar growth of MCF10A cells

Table 2 List of protein families recovered from screen colonies

total inserts	Total colonies	% of all colonies	Protein family
97	83	27.9%	Interleukin	Interleukin
97	88	29.5%	WFDC	WAP Four-Disulfide Core Domain
78	72	24.2%	FGF	Fibroblast Growth Factor
54	54	18.1%	Wnt	Wingless-type MMTV Integration Site Family
40	39	13.1%	PON	Paraoxonase
35	34	11.4%	KLK	Kalikrein
33	28	9.4%	TGFB	Transforming Growth Factor
21	19	6.4%	CXCL	CXC Chemokine Ligand
21	21	7.0%	DEF	Defensin
21	21	7.0%	FGFBP	Fibroblast Growth Factor Binding Protein
26	26	8.7%	TIMP	Tissue Inhibitor of Metalloproteinase
19	19	6.4%	CCL	CC Chemokine Ligand
19	19	6.4%	IGFBP	Insulin-like Growth Factor Binding Protein
18	18	6.0%	RSPO	R-Spondin
16	15	5.0%	DKK	Dickkopf
15	15	5.0%	SAA	Serum Amyloid
14	14	4.7%	PTHLH	Parathyroid Hormone-Like Hormone
14	14	4.7%	SFTP	Surfactant Protein
14	14	4.7%	WISP	Connective Tissue Growth Factor
13	13	4.4%	APO	Apolipoprotein
13	13	4.4%	Ly-6	Ly6/uPAR
13	13	4.4%	PGLYRP	Peptidoglycan Recognition Protein
12	12	4.0%	MASP	MBL-associated Serine Protease
10	10	3.4%	FAM3	Family with Sequence Similarity 3
10	10	3.4%	IFN	Interferon
10	10	3.4%	SPARC	Secreted Protein Acidic and Rich in Cysteine
10	10	3.4%	SUMF	Sulfatase Modifying Factor

The DNA recovered from the screen colonies was grouped in protein families, and the most prevalent protein families are listed here. As multiple protein family members may co-occur in a single colony, the number of colonies may be less than the number of inserts where the protein family is represented. Proteins that have no family members in this screen are listed as individual proteins

ISSN: 2045-3701