Figure 5
From: The Role of XPG in Processing (CAG)n/(CTG)n DNA Hairpins
Human cells possess multiple HPR pathways for nick strand hairpins. HPR assays were performed in nuclear extracts from HeLa (HL), AG08802 (AG), or GM16024 (GM) under conditions of no DNA synthesis. The resulting DNA products were digested with BglI and BsmBI, and analyzed by Southern blot analysis using an oligonucleotide probe annealing to the nicked strand near the BglI site. The repair intermediates for substrates V-(CTG)25, V-(CAG)25, C-(CTG)25, and C-(CAG)25 are shown in panels A, B, C and D, respectively.