Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13578-024-01210-y/MediaObjects/13578_2024_1210_Fig5_HTML.png)
E3 enzyme activity was required for the inhibitory effect of MARCH7 on cancer stem cells evidenced by in vivo model. A Volume change of tumor growth. B Changes in tumor weight. C Photograph of the tumors. D Western blot detected the effects of MARCH7 on NOD1 protein levels in the tumors of nude mice. E Cancer cell stemness-, proliferation-, invasion- and metastasis-, and apoptosis-related gene expression in xenograft tissues evidenced by q-PCR analysis. Here, *p < 0.01 vs. Vector group, #p < 0.01 vs. MARCH7-WT group. All experiments were conducted in triplicate, and each assay was repeated 3 times independently