Fig. 4
From: Cellular and molecular mechanisms of aspartoacylase and its role in Canavan disease
Overview of the enzymatic reactions used to assay ASPA activity. A ASPA hydrolyzes N-acetyl-L-aspartate (NAA) to acetate and aspartate. The released aspartate is coupled to other enzymatic reactions, such as B aspartate aminotransferase and malate dehydrogenase [32], C aspartase [49], and D aspartate oxidase [58]. The reactions can be followed by spectrophotometry based on the indicated specifications (red)