Fig. 7

Key examples of the application of RNA editing enzymes. A Basic strategy of endogenous ADAR1-mediated site-directed RNA editing. B Schematic diagram of the Cas13-based site-directed RNA editing system. C The CDAR-S-SNAP tool generates RNA-directed editing enzymes applying self-labeled SNAP-tag. D 3’-caged arASO light-triggered RNA editing. Cholesterol modification at the 3’ end prevents photoactivatable antisense guide RNA oligonucleotides from targeting RNA until triggered by light. E Schematic representation of the mechanism of TRIBE or PIE sequencing. The deaminase domain introduces a C-to-U or A-to-I editing sites in the sequence adjacent to the RBP binding sites. F Schematic of the READR mechanism. The sensor mRNA consists of a 5’ tag domain and a 3’ export domain separated by a 2 A coding region. Base paring between sensor mRNA and target transcript recruits ARARs, which convert the UAG stop codon to a UGG Trp codon, switching on translation of output protein