Fig. 7

BMT of C-Kit⁺-BMCs might improve Pink1-mediated mitophagy and NASH in vivo. We transplanted C-Kit⁺- or C-Kit⁻-BMCs into MCD mice. A–C Hepatic mRNA was examined by qPCR in 2 groups: A APDN, PPAR-α, FXR, LXR and SREBP-1c; B TNF-α and IL-6; C Col1a and α-SMA. D, E Hepatic protein levels of PPAR-α, FXR, α-SMA, and TNF-α were examined by western blot in 2 groups. F, G IF staining and calculation of COX-4 (green IF) and LC3B (red IF) in liver tissues of 2 groups (× 400). DAPI (blue) was used for nuclear staining. The liver mRNA H and protein I, J levels of Pink1-mediated mitophagy pathway were examined by qPCR and western blot in 2 groups. The p-value indicated statistical significance compared to MCD_C-Kit⁻-BMC mice