Fig. 5

C-Kit⁺-LSECs improve the mitochondrial function of HCs in vitro. A–D pHCs were cocultured with PA-treated C-Kit⁺- or C-Kit⁻-pLSECs. IF staining and calculation of LC3B (red IF) and COX4 (green IF, A/B), mtSOX (red IF, A/C), and mtKeima (red IF, A/D) in pHCs (× 400). DAPI (blue) was used for nuclear staining. E–G HepG2 cells were cocultured with 6 groups of TMNK-1 cells. E, F IF staining and calculation of LC3B (red IF) and COX4 (green IF) in HepG2 cells (× 500). DAPI (blue) was used for nuclear staining. G The mRNA of mitophagy-related genes (PINK1, Parkin, LC3B) was examined by qPCR. The p-value indicated statistical significance compared to the C-Kit⁻-pLSEC, BSA, sh-NC + PA or ov-NC + PA group