Fig. 8

PSMD7 oncogenesis depends on the SOX2-Notch1 pathway. (A) Expression of HES1, Notch1, and PSMD7 in PC cells that were co-transfected with SOX2 and sh-PSMD7. (B) and (C) EdU and CCK-8 analysis of cell proliferation in the PANC-1 cells that express shPSMD7 or shNC, with or without overexpression of SOX2. (D) Expression of HES1, Notch1, and PSMD7 in PC cells that were co-transfected with sh-SOX2 and Flag-PSMD7. (E) and (F) EdU and CCK-8 analysis of cell proliferation in SW 1990 cells that express exogenous PSMD7 or control vector, with or without the transfection of sh-SOX2. (G) Mice injected with luciferase-expressing PANC-1/SOX2, PANC-1/shPSMD7, PANC-1/shNC, or PANC-1/shPSMD7 + SOX2 cells were examined through IVIS imaging system. (H) Tumour volumes in the PANC-1/SOX2, PANC-1/shPSMD7, PANC-1/shNC, or PANC-1/shPSMD7 + SOX2 group. Volumes of tumours are expressed as the mean ± SD, n = 6, **p < 0.01. (I) Tumour weight in the PANC-1/SOX2, PANC-1/shPSMD7, PANC-1/shNC, or PANC-1/shPSMD7 + SOX2 group. n = 6. **p < 0.01. (J) Suggested mechanistic scheme for PSMD7 to facilitate the Notch1 signalling pathway mediated by SOX2 in PC, r = 0.3649, p = 0.0067. *p < 0.05, **p < 0.01