Fig. 6

PSMD7 interacts with SOX2. (A) and (B) qRT-PCR analysis of PSMD7 and SOX2 mRNA expression in PANC-1 cells transfected with either shNC or shPSMD7, and SW 1990 cells transfected with exogenous PSMD7 or a control vector. n = 3; ns, not significant. **p < 0.01. (C) The first 5 proteins that co-precipitated with PSMD7 were identified via LC-MS/MS. #PSMs with matching peptide profiles. (D) Mass spectra displaying distinct peptides of PSMD7 characterized by 2D-LC-MS/MS after immunoprecipitation of PANC-1 cell lysates with anti-PSMD7. (E) and (F) Immunoprecipitation of SW 1990 and PANC-1 cell lysates with control IgG, anti-SOX2, or anti-PSMD7 antibodies. (G) SW 1990 and PANC-1 cells were fixed and stained using SOX2 (green) and PSMD7 (red) antibodies. Cell nuclei were stained using DAPI (blue). Scale bar: 20 μm. (H) Top ranked docking conformations and 3D structures of SOX2 and PSMD7. SOX2 and PSMD7 are displayed in cyan and green, respectively. (I) Schematic illustration of SOX2 structure. (J) Diagram of SOX2 truncated mutant constructs. (K) HMG region of SOX2 was required for interaction with PSMD7. *p < 0.05, **p < 0.01