Fig. 3

Overexpression of PSMD7 promotes PC proliferation. (A) and (B) PSMD7 mRNA along with protein levels in SW 1990 cells transfected with Vector or Flag-PSMD7 plasmids were detected by qRT-PCR as well as western blotting. Tubulin was employed as the loading control. (C) CCK-8 assay was employed to examine the difference in cell viability between both groups. (D) and (E) EdU staining was utilized to establish the impact of PSMD7 overexpression on the PC cell proliferation rate. Scale bar denotes 50 μm, **p < 0.01. (F) Mice injected with luciferase-expressing SW 1990/Flag-PSMD7 or SW 1990/Vector cells were investigated through IVIS imaging system. (G) Tumour volumes for SW 1990/Flag-PSMD7 or SW 1990/Vector groups. Volumes of tumours are expressed as mean ± SD, n = 6, **p < 0.01. (H) Tumour weights in the Flag-PSMD7 and Vector groups, n = 6, **p < 0.01. (I) Tumour volumes for SW 1990/Flag-PSMD7 or SW 1990/Vector groups. (J) and (K), immunohistochemistry (IHC) analysis of Ki-67 and PSMD7 expression in tumours of the SW 1990/Flag-PSMD7 or SW 1990/Vector group, scale bar is 20 μm. n = 3, *p < 0.05, **p < 0.01