Fig. 6

MARCH7 reversed NOD1 induced CSCs stem-like properties. A Western blot determined the effects of MARCH7 on the NOD1 protein levels in T24^CSCs and 253J^CSCs. B, C Overexpressed MARCH7 reverses the effects of NOD1 on spheroid formation and spheroid size of T24^CSCs and 253J^CSCs, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. D MARCH7 reverses the effects of NOD1 on the expression of stem-like markers in T24^CSCs and 253J^CSsC detected by immunoblotting, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. E MARCH7 reverses the effects of NOD1 on the proliferation of T24^CSCs and 253J^CSCs detected by CCK-8 assay, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. F, G MARCH7 reverses the effects of NOD1 on colony formation of T24^CSC and 253J^CSC, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. H The effect of NOD1 and MARCH7 on the expression of proliferation related proteins in T24^CSCs and 253J^CSCs, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. I, J Transwell assay detected the effect of NOD1 and MARCH7 on the invasion of T24^CSCs and 253J^CSCs, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. K Effects of NOD1 and MARCH7 on the expression of invasion x-related genes in T24^CSC and 253J^CSC, *p < 0.01 vs. Vector group, ^#p < 0.01 vs. NOD1 group. D and H The loading control, tublin-α is the same band, as the data from the same membrane. All experiments were conducted in triplicate, and each assay was repeated 3 times independently