Fig. 3

MARCH7 reduces bladder CSCs proliferation, colony formation and invasion. A The effects of up-regulated MARCH7 on proliferation of T24 and 253 J derived stem-like cells shown by CCK-8 assay, *p < 0.01 vs. Vector group. B, C Colony formation in stem-like cells transfected with MARCH7, *p < 0.01 vs. Vector group. D MARCH7 modulated proliferation proteins in CSCs, *p < 0.01 vs. Vector group. E, F Transwell assay detected the effects of MARCH7 on invasion of T24^CSCs and 253J^CSCs, *p < 0.01 vs. Vector group. G Up-regulation of MARCH7 affected invasion genes in T24^CSC and 253J^CSC, *p < 0.01 vs. Vector group. D and G The loading control, tublin-α is the same band in Fig. 2F, as the data from the same membrane. All experiments were conducted in triplicate, and each assay was repeated 3 times independently