Fig. 2

MARCH7 inhibited the stem-like capacities of bladder. A, B q-PCR and western blot detecting MARCH7 in CSCs (T24^CSC and 253 J^CSC), *p < 0.01 vs. vector group. C–E The effect of MARCH7 on the spheroid formation ability and spheroid size, and spheroid number of T24^CSC and 253J^CSC. F The effect MARCH7 on stem-like markers in T24^CSC and 253J^CSC, *p < 0.01 vs. vector group. G Flow cytometry assay detect stem-like markers in T24^CSC and 253J^CSC when overexpressed MARCH7. All experiments were conducted in triplicate, and each assay was repeated 3 times independently