Fig. 1

(A) Uniform Manifold Approximation and Projection (UMAP) Visualization of the Clustering of All 11350 Nuclei sequenced from All Patient Samples, (B) UMAP Visualization of Cell Population from Individual Samples and (C) Dotplot for Cell Type Identification Markers Each dot in A & B represents one nucleus, with its position differentiated by the expression levels of the principal components. 14 clusters were isolated in total and were identified to be the major intestinal cell types based on markers shown in C. There was no emergence of novel cell subtypes based on diseased conditions and the differences between the cell populations of all the samples can be characterized by the change of population compositions. For example, the advanced decompensated patient had more lymphocytes, transit-amplifying cells and less enterocytes compared to other samples with less severe conditions