Table 3 Summary of parameters extracted by analyzing ATR-FTIR and solution SAXS spectra of α-synuclein fibril, Aβ-42 fibril and modified and un-modified protein solutions of LGAB and BSA
Sample | Vibrational frequency peak center of β-sheet/ aggregated structure (cm−1) | % β-sheet | Particle shape | \({R}_{g}\)(nm) | a (nm) | b (nm) |
|---|---|---|---|---|---|---|
α- synuclein fibril- pH 7.4 | 1630 | 51 ± 2 | Elliptical cylinder | 3.5 ± 0.05 | 13.3 ± 0.2 | |
Aβ-42 fibril-pH 7 | 1626 | 55 ± 2 | Elliptical cylinder | 3.0 ± 0.08 | 9.2 ± 0.1 | |
LGAB fibril-pH 2 | 1624 | 54 ± 3 | Elliptical cylinder | 1.1 ± 0.03 | 9.8 ± 0.09 | |
BSA fibril-pH 2 | 1617 | 33 ± 5 | Elliptical cylinder | 1.4 ± 0.2 | 10.4 ± 0.4 | |
LGAB-pH 2 | 1628 | 54 ± 2 | Prolate ellipsoid | 2.3 ± 0.05 | 1.5 ± 0.01 | 3.2 ± 0.02 |
LGAB-pH 7 | 1628 | 55 ± 4 | Prolate ellipsoid | 2.2 ± 0.01 | 1.3 ± 0.05 | 3.3 ± 0.04 |
BSA-pH 2 | 1622 | 20 ± 3 | Prolate ellipsoid | 3.0 ± 0.02 | 1.3 ± 0.02 | 4.6 ± 0.02 |
BSA-pH 7 | 1622 | 18 ± 2 | Prolate ellipsoid | 2.9 ± 0.01 | 1.4 ± 0.01 | 4.5 ± 0.02 |