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Fig. 5 | Cell & Bioscience

Fig. 5

From: Synthetic β-sheets mimicking fibrillar and oligomeric structures for evaluation of spectral X-ray scattering technique for biomarker quantification

Fig. 5

sSAXS calibration using amyloid-like oligomers of LGAB and BSA with % β-sheets of 55 and 18 respectively. sSAXS spectra of unmodified a LGAB and d BSA oligomer and b, e their 0.4/0.6 (40% oligomer and 60% PMMA) blends with tissue mimicking polymethyl methacrylate (PMMA). c sSAXS spectrum of pure PMMA powder. Spectra of oligomers and PMMA are deconvolved using Gaussian curves. Raw data are shown with symbols and the cumulative fits are shown with black lines. In (c) for pure PMMA, a peak center at q = 6.3 nm−1 is deliberately inserted during deconvolution and the resultant curve indicates the absence of corresponding peak in the raw data. f Area under the peak of q = 6.3 nm−1 (\({AUP}_{q=6.3 {nm}^{-1}}\)) as a function of oligomer weight fraction (\({f}_{oligomer}\)) for BSA and LGAB. For each oligomer weight fraction, three independent measurements were performed on freshly prepared samples and the error bars in (f) are estimated based on that

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