Fig. 6

PVRL4-decifient mice are more sensitive to viral infection. (A-C) BMDMs derived from WT and Pvrl4^−/− mice were infected with VSV (MOI = 1), HSV-1 (MOI = 10) and IAV (MOI = 5). And the viral M RNA level of VSV were measured by qRT-PCR (A), the luciferase activity of HSV-1 was measured (B) and the IAV NP vRNA, mRNA and cRNA levels were measured by qRT-PCR (C). (D and E) WT and Pvrl4^−/− mice were intraperitoneally injected with VSV (1 × 10⁸PFU/g) (n = 4). The VSV RNA expression in organs was detected by qRT-PCR after infection for 36 h (D). The lungs were stained with H&E (E). Scale bar, 100 μm; inset scale bar, 100 μm. (F) H&E staining of lungs of WT and Pvrl4^−/− mice infected with 10,000 PFU of IAV at 2 days post-infection. Scale bar, 100 μm; inset scale bar, 100 μm. (G) Immunohistochemical detection of NP in the lungs of WT and Pvrl4^−/− mice infected with IAV at 2 days post-infection. Scale bar, 50 μm; inset scale bar, 50 μm. (H-J) 6 to 8 weeks mice of WT and Pvrl4^−/− were infected with IAV (10000PFU) intranasally. The RNA level of NP in the lung were measured by qRT-PCR at 2 and 4 days post-infection (H, n = 4). And the bodyweight loss (I) and survival (J) were monitored for 8 days and 14 days, respectively (n = 15)