Fig. 3

Elevated viral infection in PVRL4-deficient cells. (A-E) WT and PVRL4^−/−HEK293T cells were infected with VSV (MOI = 0.001), and the GFP in the cells were visualized by fluorescence microscopy (A). The GFP-positive cells were analyzed by flow cytometry (B), and values calculated and presented in (C). The viral M RNA level (D) and the vitral titers (E) were measured by qRT-PCR and plaque assay, respectively, for the indicated times. Scale bar, 100 μm. The “Lg” means “Log₁₀ change”. (F-J) WT and PVRL4^−/−HEK293T cells were infected with HSV-1 (MOI = 0.001). The GFP in the cells were measured by fluorescence microscopy (F). The GFP-positive cells were analyzed by flow cytometry (G), and values calculated and presented in (H). And the luciferase activity was measured and normalized to the WT (I). And after 48 h infection, the vitral titers in supernatants were determined by plaque assay (J). Scale bar, 100 μm. (K-N) WT and PVRL4^−/−HEK293T cells were infected with IAV (MOI = 0.001), and the viral NP vRNA, mRNA and cRNA level (K-M) and viral NP protein expression (N) were measured by qRT-PCR and western blotting assay, respectively, for the indicated times. Data are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001