Fig. 1

PVRL4 is induced by IFN treatment and viral infection. (A and B) PVRL4 expression was measured by qRT-PCR in WT BMDMs after treatment with recombinant mouse IFN-α (1000U/mL) (A), or IFN-β (1000U/mL) (B) for the indicated times. (C-E) qRT-PCR and western blotting analysis of PVRL4 expression in WT and Ifnar1^−/− BMDMs treated with mouse IFN-α (500U/mL) or IFN-β (500U/mL). (F-I) qRT-PCR and western blotting analysis of PVRL4 expression in WT and IFNAR1^−/− A549 cells infected with VSV, HSV-1 and IAV for the indicated times. Mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001