Fig. 6
From: Tunable PhenoCycler imaging of the murine pre-clinical tumour microenvironments
PhenoCycler imaging of the murine melanoma tumour microenvironment. (A) Schematics of the BRAFV600E/PTEN−/− and YUMMER1.7 murine models of melanoma. (B) Heatmap showing the normalized cellular mean intensity of markers within the PhenoCycler staining panel, in different phenotypes of cells in BRAFV600E/PTEN−/− and YUMMER1.7 tumours. (C) Normalized Ki67 mean fluorescence intensity in Ki67 + tumour cells and Ki67- tumour cells. (D) Proportions of different cell types in BRAFV600E/PTEN−/− tumours. (E) Heatmap showing neighborhood analysis of BRAFV600E/PTEN−/− tumours, as Pearson correlation coefficient between cells. (F) Representative image of BRAFV600E/PTEN−/− tumour core. (G) Proportions of different cell types in YUMMER1.7 tumours, treated with IgG control or αPD-1. (H-I) Heatmap showing neighborhood analysis of YUMMER1.7 tumours treated with IgG control (H) or αPD-1 (I), as Pearson correlation coefficient between cells. (J) Representative image of YUMMER1.7-IgG tumour core. (K) Representative image of YUMMER1.7-αPD-1 tumour core