Fig. 5

PhenoCycler imaging of the murine breast cancer tumour microenvironment. (A) Schematic of the 66cl4 murine model of breast cancer. (B) Schematics of the 4T1 murine models of breast cancer and breast cancer liver metastasis. (C) Heatmap showing the normalized cellular mean intensity of markers within the PhenoCycler staining panel, in different phenotypes of cells in 66cl4 and 4T1 tumours. (D) Representative image showing Ki67 + and Ki67- tumour cells. (E) Proportions of different cell types in 66cl4 and 4T1 tumours. (F) Representative images of macrophages and T cells in 66cl4 and 4T1 primary tumours. (G) Heatmap showing neighborhood analysis of 66cl4 tumours. as Pearson correlation coefficient between cells. (H) Heatmap showing neighborhood analysis of 4T1 primary tumours, as Pearson correlation coefficient between cells. (I) Representative images showing immune cell infiltration patterns in 66cl4 and 4T1 tumours. (J) Heatmap showing neighborhood analysis of 4T1 liver metastases, as Pearson correlation coefficient between cells. K. Representative images comparing immune and stromal cell types in 4T1 primary and 4T1 liver metastases