Fig. 4

PhenoCycler imaging of the murine nodal and extra-nodal B-cell lymphoma tumour microenvironment. (A) Schematic of the A20 model of extranodal B-NHL, and representative images of Ki67 staining in healthy liver and A20 tumour-bearing liver. (B) Schematic of the Eµ-Myc model of nodal B-NHL, and representative images of Ki67 staining in healthy lymph node and an Eµ-Myc tumour-bearing lymph node. (C) Heatmap showing the normalized cellular mean intensity of markers within the PhenoCycler staining panel, in different phenotypes of cells in A20 and Eµ-Myc tumours. (D) Scatter plot comparing the proportions of different cell phenotypes as determined by PhenoCycler staining versus archival flow cytometry data, for A20 tumours. Pearson r = 0.8551, p = 0.0142. (E) Proportions of different cell types in adjacent healthy liver and A20 tumour-bearing liver. (F) Heatmap showing neighborhood analysis of A20 tumours, as Pearson correlation coefficient between cells. Blue hue indicates cells are likely to be in further proximity, while red hue indicates that cells are likely to be in closer proximity. (G) Representative image of an A20 tumour core. (H) Proportions of different cell types in healthy lymph nodes and Eµ-Myc tumour-bearing lymph nodes. (I) Heatmap showing neighborhood analysis of Eµ-Myc tumours, as Pearson correlation coefficient between cells. (J) Representative image of an Eµ-Myc tumour core